Optimization and evaluation of luminex performance with supernatants of antigen-stimulated peripheral blood mononuclear cells

HIGHLIGHTS

• who: Mathieu Surenaud from the INSERM, Equipe, Créteil, France have published the research work: Optimization and evaluation of Luminex performance with supernatants of antigen-stimulated peripheral blood mononuclear cells, in the Journal: (JOURNAL)
• what: The aim of this study was to optimize a cell culture protocol (including quantity of cells, culture duration, and positive control) to obtain reproducible cytokine and chemokine quantifications by Luminex technology in antigen-stimulated PBMC supernatants; the second aim was then to compare the performances of different Luminex kits. The authors focused on four cytokines: the T-helper (Th)1 . . .
  

   

  

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