HIGHLIGHTS
SUMMARY
To the traditional physical and chemical methods, isothermal titration calorimetry, surface plasmon resonance, microscale thermophoresis (MST), and biolayer interferometry have been recently developed to investigate the interaction between aptamers and small_molecule ligands, as well as to measure the main kinetic or thermodynamic parameters. Researchers are interested in the aptamer-based SERS method, which mainly fixes the modified aptamer on the surface of nanoparticles or matrix, constructs a SERS biosensor to study the interaction between small_molecules and aptamers, as well as achieves accurate quantitative analysis. The SERS the band 1487 of cm -1 increased gradually . . .
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