HIGHLIGHTS
SUMMARY
This means that interpreting the effect of an amino_acid residue should not be inferred from a single peptide (one data point in the ranking) but rather using groups of peptides all having the same amino_acid residue at a specific position (multiple data points in the ranking). To robustly measure the effect of a particular amino_acid residue on antimicrobial activity, the authors investigated whether specific amino_acid residues were significantly enriched in peptides with a higher or lower antimicrobial activity using a permutation scheme (Fig 1b; enrichment calculation is explained in more depth for Fig 2a . . .
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