Origin, specification and differentiation of a rare supporting-like lineage in the developing mouse gonad

HIGHLIGHTS

• who: Chlou00e9 Mayu00e8re et al. from the Histological and IF analyses Embryonic or postnatal samples were collected at specific time points determined by the day of vaginal plug (E05) and fixed overnight at, u00b0C in, % paraformaldehyde. Samples were embedded in paraffin, and, uf06dm sections were prepared. Unmasking was performed in TEG (, mM, pH, ) or citrate (, mM, pH, ) buffer for , min in a pressure cooker. Sections were incubated in blocking buffer [3% bovine serum albumin (BSA) and, .1% Tween in phosphate-buffered saline (PBS)] for , hours at room temperature. Primary antibodies were incubated overnight at, u00b0C. Secondary antibodies . . .
  

   

  

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