Original article

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  • who: Phoenix dactylifera Date palm and colleagues from the Thionin gene manipulation A prepared recombinant plasmid (pMiniT) containing the thio, gene was supplied from Faculty of Science, Helwan UniversityThe plasmid was conserved in DH5-u03b1 bacterial glycerol stock. A bacterial sub-culture was performed for amplification of the recombinant plasmid on LB broth media supplemented with ampicillin antibiotic (, u00b5g/ ml). A plasmid mini-prep protocol was applied to isolate the modified plasmid using alkaline lysis method according to the protocol of Maniatis et_al (1982). have published the article: ORIGINAL ARTICLE, in the Journal: (JOURNAL)
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