Osteopontin regulates actin cytoskeleton and contributes to cell proliferation in primary erythroblasts*

HIGHLIGHTS

• who: Jeong-Ah Kang from the serum-free media (IMDM/1% bovine serum albumin) for , h prior to stimulation with OPN. Immunoblot analysis was performed as described previously (16). An ELISA for OPN was performed using medium collected from cultures where cells had been seeded at a concentration of , ⫻, ml. After, days of culture, the medium was collected and subsequently used in the ELISA assay. Also, an OPN ELISA was performed using a commercially available kit according to the manufacturer's instructions (Assay Design, Inc.). Isolation and Culture of Mouse Erythroblasts from OPN⫺/⫺ Mice-Mice (B6.Cg . . .
  

   

  

  If you want to have access to all the content you need to log in!

  Thanks :)

  Username or Email

  Password

  Remember me

  Lost your password?

  If you don't have an account, you can create one here.