Overlapping but separable determinants of dna binding and nuclear localization map to the c-terminal end of the caenorhabditis elegans daf-12 dna binding domain*

HIGHLIGHTS

• who: Yuriy Shostak from the Yeast Transcriptional Reporter Assay-Celegans , response element-containing, ., and, ., genomic fragments (32) were subcloned upstream of a minimal , promoter driving LacZ expression in pdSS (2␮, URA-marked) vector (33) (plasmids pYSYR0001-3). Expression of, terminally FLAG- or GFP-tagged (with or without , NLS) wild type , (aa, ) and mutants (in order of appearance in Figs., and, ) was driven by the copper-inducible , promoter in p, (2␮, TRP) (34) vector, in the presence ofmM Cu, (plasmids p, and pYSYE0101-118, respectively). The plasmids were transformed (35) into Saccharomyces cerevisiae, a strain. Expression assays and . . .
  

   

  

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