Oxidative stress in the mitochondrial matrix underlies ischemia/reperfusion-induced mitochondrial instability

HIGHLIGHTS

• who: Soroosh Solhjoo from the Monolayer cultures of neonatal rat ventricular myocytes NRVMs were isolated from, day-old Sprague-Dawley rats (Harlan Laboratories) as described previously (11)All experimental procedures were approved by the Institutional Animal Care and Use Committee of Johns Hopkins University. One million NRVMs were cultured on each fibronectin-coated circular cover glass (D=, mm) and superfused with a medium containing, % fetal bovine serum. The medium was changed every day. After , to , days of culture, confluent monolayers were used for IR experiments. Fluorescent dye and molecular probes To measure oxidative stress, pH-insensitive ratiometric . . .
  

   

  

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