Pacap-pac1r modulates fear extinction via the ventromedial hypothalamus

HIGHLIGHTS

  • who: E. R. Velasco from the Universitat University Pompeu have published the paper: PACAP-PAC1R modulates fear extinction via the ventromedial hypothalamus, in the Journal: NATURE COMMUNICATIONS NATURE COMMUNICATIONS
  • what: The authors used a non-PACAP-selective combined chemogenetic retrograde AAV approach to inhibit the activity of this circuit by injecting AAV-hM4Di-DREADD (AAV8-hSyn-DIOhM4D(Gi)-mCherry) or AAV-DIO-mCherry (AAV8-hSyn-DIOmCherry) and AAV5rg-pmSyn-EBFP-Cre, so that hM4D(Gi) receptors were expressed in VMHdm-projecting MeA neurons or MeA-projecting VMHdm neurons (Fig 4a, c). mCherry and enhanced blue . . .

     

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