Pan-cancer emt-signature identifies rbm47 down-regulation during colorectal cancer progression

HIGHLIGHTS

• who: Matjaz Rokavec from the Cell lines, cell culture and reagentsColorectal cancer (SW480, SW620, Caco-2), breast cancer (MCF7, MDA-MB-231), and prostate cancer (LNCaP, PC-3) cell lines were maintained in Dulbecco's Modified Eagles Medium (DMEM, Invitrogen) containing, % fetal bovine serum (FBS, Invitrogen). The colorectal cancer cell lines HCT-15, HT29, LS T, and , were maintained in McCoy's , A Medium (Invitrogen) containing, % FBS. All cells were cultivated in presence of , units/ml penicillin and, ., mg/ml streptomycin. SiRNAs (Ambion silencer siRNA: negative control (ID#4611) and , (ID#s29090) were transfected at a final . . .
  

   

  

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