Paracrine secretion of il8 by breast cancer stem cells promotes therapeutic resistance and metastasis of the bulk tumor cells

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  • who: Mingming Wu from the The MCF-7, T D and MDA-, cells were purchased from , cells were obtained from Professor Suling Liu (Fudan University, China). HEK T cells were obtained from Dr. Huanfeng Zhang (USTC). Cells were cryopreserved soon upon receipt and continuously cultured for less than , months. MCF-7, T D and MDA-, cells have been authenticated by STR genotyping. No cross-contamination with other human cells was observed. Possible mycoplasma contamination of all cell lines is routinely and regularly monitored. , and T D cells were cultured in RPMI , (Gibco, California, America) supplemented with, % fetal . . .

     

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