Paraquat toxicity induced by voltage-dependent anion channel 1 acts as an nadh-dependent oxidoreductase*

HIGHLIGHTS

• who: Hiroki Shimada from the Preparation of Mitochondria Mitochondria were isolated from the livers of male Wistar rats or from HeLa cells by differential centrifugation (11, )Mitochondria were suspended inM sucrose solution containingM Tris-HCl, mM KCl, ., mM MgCl2, and, ., mM Na , (pH, .4). The mitochondria were starved for , min at , °C to consume endogenous substrates before use. The Kanazawa Medical University Animal Care and Use Committee approved all studies. All animals were cared for and treated in accordance with the Committee guidelines. PQ-dependent Hydrogen Peroxide (H, ) Production on Mitochondria Mitochondria were attached onto a glass . . .
  

   

  

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