Pax-6 and cdx-2/3 interact to activate glucagon gene expression on the g1 control element*

HIGHLIGHTS

• who: Beate Ritz-Laser from the Cell Culture and DNA Transfection-The glucagon-producing hamster In, (13) and the non-islet Syrian baby hamster kidney (BHK21) cell lines were grown in RPMI , (Seromed, Basel, Switzerland) supplemented with, % heat-inactivated fetal calf serum and, % heatinactivated newborn calf serum, mM glutamine, units/ml of penicillin, and , mg/ml of streptomycinInR , cells were generously provided by Dr. R. Takaki (Medical College of Oita, Oita, Japan). , cells were transfected by the calcium phosphate precipitation technique (14) using , mg of total plasmid DNA/10-cm Petri dish. , mg of pSV A pap . . .
  

   

  

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