Pentosan polysulfate as an inhibitor of extracellular hiv-1 tat*

HIGHLIGHTS

• who: Marco Rusnati from the Reagents-Recombinant , Tat (86-amino acid isoform) was expressed in Escherichia coli as glutathione, transferase (GST) fusion protein (9)GSTu4860Tat was also fused at its C terminus to the green fluorescent protein (GFP) (14). The GST and GFP moieties do not interfere with LTR-transactivating activity and heparin-binding capacity of Tat (13, ). Pentosan polysulfate was from Sigma (St Louis, MO). Heparin was from Laboratori Derivati Organici SpA, (Milan, Italy). u2424-Cyclodextrin tetradecasulfate was from Glycores , (Milan, Italy). Fluorescein-labeled bovine serum albumin (FITC-BSA) was kindly provided by Prof. S. Marchesini . . .
  

   

  

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