Peripheral membrane proteins modulate stress tolerance by safeguarding cellulose synthases

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  • who: S C I E and colleagues from the Plasmid constructs A genomic fragment spanning the, kb , promoter region upstream of the start codon and the , genomic region without stop codon was polymerase chain reaction (PCR) amplified using the primers detailed in table , and cloned into the pENTR/, TOPO vector (Invitrogen). The CESA1-C coding sequence for expression in N. benthamiana was PCR amplified using specific primers using pGBKT7-CesA1-C domain as the template and the att, adapter universal primer (Invitrogen) and introduced into the pDONR/Zeo vector (vector) using the BP Clonase II Gateway Cloning . . .

     

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