Phage-like particle vaccines are highly immunogenic and protect against pathogenic coronavirus infection and disease

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• who: Bennett J. Davenport from the Purification of recombinant hCoV-RBD proteins A pCAGGS expression vector encoding the SARS-Co, spike RBD was obtained from DrFlorian Krammer, Icahn School of Medicine at Mount Sinai93. This construct contains sequences encoding the native signal peptide (residues, ) followed by residues, from the SARSCo, Wuhan-Hu, spike protein (GenBank MN908947.3) and a hexahistidine tag at the, terminus for purification. A pTwist-CMV expression vector (Twist Biosciences Technology) encoding the MERS-CoV spike RBD was kindly provided by Dr. Peter S. Kim, Stanford University94. This construct contains sequences encoding an, terminal . . .
  

   

  

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