Phage t4 dna [n]-adenine6methyltransferase. overexpression, purification, and characterization ∗

HIGHLIGHTS

  • who: Valeri G. Kossykh from the Valeri GKossykh:j:, Samuel L. Schlagman, and Stanley Hattman From the Department of Biology, University of Rochester, Rochester, New York , have published the research: Phage T4 DNA [N]-adenine6Methyltransferase. OVEREXPRESSION, PURIFICATION, AND CHARACTERIZATION ∗, in the Journal: (JOURNAL) of February/15,/1995

SUMMARY

    The bacteriophage T4 dam gene, encoding the Dam DNA methyltransferase (MTase), has been subcloned into the plasmid expression vector, pJW2. T4 Dam methylates the palindromic tetranucleotide, GATe, designated the canonical sequence. At high MTase:DNA ratios, T4 Dam can methylate some noncanonical sequences belonging to . . .

     

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