HIGHLIGHTS
SUMMARY
To improve the efficiency of substitution, a number of BE technologies have been developed (Komor et_al, 2016; Nishida et_al, 2016; Gaudelli et_al, 2017; Anzalone et_al, 2019). Most BE technologies are based on an engineered Cas9 protein fused with a single strand DNA-specific cytidine deaminase (CDA; Komor et_al, 2016; Nishida et_al, 2016; Nishimasu et_al, 2018). Target-AID base-editing technology, in which an Arabidopsis thaliana-optimized codon nickase Cas9 (nCas9) fused with a lamprey CDA is used, has been demonstrated as Frontiers in Plant Science | www.frontiersin.org July 2022 | Volume 13 | Article 848560 . . .
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