HIGHLIGHTS
- who: Travis J. MacLeod from the Materials-Primary human umbilical vein endothelial cells were a generous gift from DrK. Patel (University of Calgary). HEK , cells were obtained from American Type Tissue Culture collection. Gluplasminogen and plasmin were purchased from American Diagnostica. The wild-type annexin, wild-type S, and S, desKK deletion mutant (lacking the last two lysine residues of the carboxyl terminus) were expressed in Escherichia coli and purified as described previously (28). This recombinant annexin , was used in the binding experiments. AIIt and other annexins were purified from bovine lung as described in Khanna et_al . . .
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