Phosphorylation of eukaryotic initiation factor-2α (eif2α) in autophagy

HIGHLIGHTS

• who: Juliette Humeau from the Human osteosarcoma U OS were purchased from the ATCCU OS cells stably expressing GFP-, and , were generated by our group in the past27, . MEF wild type (WT) and knockout for the four eIF2α kinases (MEF KO) were provided by Professor Seiichi Oyadomari from Tokushima University. MEF WT RFP-, and KO RFP-, were constructed from the aforementioned cell lines, which were transduced with LentiBright lentiviral particles coding for RFP-, (17-10143, Millipore, Burlington, MO, USA). The day following transduction, cells were supplemented with fresh medium and, day later, single cell sorted by flow . . .
  

   

  

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