Pitx factors are involved in basal and hormone-regulated activity of the human prolactin promoter*

HIGHLIGHTS

• who: Marie-He̕le̠ne Quentien from the Plasmid Constructs and Mutagenesis-The reporter plasmid hPRL164luc was previously described (15)Human Pitx, was provided by Dr. D. A. Clayton (Stanford University, CA). Human Pitx, isoform a and Pit, full-length cDNA coding regions were cloned by PCR using normal pituitary tissues and specific oligonucleotide sequences, and subcloned into the CMV-driven eukaryotic expression vector pc, (Invitrogen). The mutations of the , and , binding sites in the hPRL-164luc construct were as described elsewhere (15). Mutations of the, sites in the hPRL-164luc construct, the R P and R . . .
  

   

  

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