Plant genome editing with talen and crispr

HIGHLIGHTS

• who: Aimee Malzahn from the the DSB means that any indels will likely fail to disrupt the PAM site, leaving the possibility for multiple Cpf, targeting events and allowing a second chance for gene insertion should the first attempt failReports of Cpf, in plants have also been published recently [116-121]. The CRISPR-Cpf, system developed by Tang et_al achieved, % mutagenesis frequency at all target sites in rice [119], demonstrating promising applications of Cpf, in plants. DNA independent delivery of SSNs for plant genome editing is another trend. Development of such methods are likely motivated for use . . .
  

   

  

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