Polar mutagenesis of polycistronic bacterial transcriptional units using cas12a

HIGHLIGHTS

  • who: Antoine Graffeuil from the (UNIVERSITY) have published the paper: Polar mutagenesis of polycistronic bacterial transcriptional units using Cas12a, in the Journal: (JOURNAL)
  • what: The authors aimed to test whether sequences with such a tendency to generate secondary_structures could be readily engineered in the E. coli chromosome using CRISPR/Cas technologies. The aim of the following study was to design a strategy to shut down the transcription of entire polycistronic operons using synthetic terminator sequences in E. coli. For this experiment, the authors compared the wild_type strain with the mutant that has an L3S2P56 insertion . . .

     

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