Polyamine-mediated apoptosis of alveolar macrophages during pneumocystis pneumonia*

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• who: Mark E. Lasbury from the Rodent Models of Pcp-Pneumocystis organisms for transtracheal inoculation were obtained from previously infected Sprague-Dawley rats or BALB/c miceLungs of infected animals were homogenized in a Tenbroeck homogenizer (Fisher). The homogenate was centrifuged for , min at , ⫻ g to remove large aggregates. The clarified supernatant was adjusted to , trophozoites per, ⫻ oil immersion field and used as the inoculum. Each immunosuppressed rat was transtracheally instilled with , ␮l of this inoculum (40), whereas each mouse received , ␮l of inoculum with of , trophozoites per, ⫻ oil immersion field. The unused inoculum was slowfrozen in . . .
  

   

  

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