Popsicle: an all-optical reporter system for synaptic vesicle recycling combining ph-sensitive fluorescent proteins with optogenetic manipulation of neuronal activity

HIGHLIGHTS

• who: Marius Seidenthal from the Raffaele University, Italy have published the research work: pOpsicle: An all-optical reporter system for synaptic vesicle recycling combining pH-sensitive fluorescent proteins with optogenetic manipulation of neuronal activity, in the Journal: (JOURNAL)
• what: The authors characterize two different combinations of ChRs with pH-sensitive fluorescent proteins in living C. elegans nematodes.
• how: For increased ChrimsonSA stimulation at 280 u00b5W/mm2 the authors used the same excitation filter as for pHuji experiments but combined with a FITC/TxRed double band pass beamsplitter and a 515-555 nm emission . . .
  

   

  

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