Preimplantation factor (pif) correlates with early mammalian embryo development-bovine and murine models

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  • who: Stamatkin et_al Reproductive Biology et al. from the The study has been approved by the Louisiana State UniversityAt the Louisiana State University Embryo Biotechnology Laboratory, in vitro fertilization (IVF) was performed on bovine oocytes obtained from a commercial source (Ovitra, TX). Oocytes arrived in a climate controlled container via FedEx at ~, hours following their collection. A standard bovine IVF laboratory procedure was performed on groups of , oocytes in , μl droplets of fertilization medium (IVF-TALP). Briefly, μl of heparin (, μg/ml), μl of PHE and , μl sperm were added to each fertilization droplet with the, II . . .

     

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