Prevalence and whole genome-based phylogenetic, virulence and antibiotic resistance characteristics of nasal staphylococcus aureus in healthy swiss horses

HIGHLIGHTS

  • who: Schlu00fcsselwu00f6rter Antibiotika and collaborators from the WGS libraries were prepared from DNA extracted from colonies grown on TSA-SB agar plates overnight at, u00b0C using the NexteraTM DNA Flex microbial colony extraction protocol (Illumina Inc [, ]San Diego, CA, US). Prior to library preparation, the DNA was purified using the AMPure XP magnetic beads (Beckman Coulter, Brea, CA). Libraries were prepared with the Nextera DNA Flex Library Prep Kit following the manufacturer`s instructions (Illumina), and sequenced (2x, bp paired-end) on an Illumina MiSeq platform at the Next Generation Sequencing Platform, Institute of Genetics, Vetsuisse Faculty . . .

     

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