Profilin-1 deficiency leads to smad3 upregulation and impaired 3d outgrowth of breast cancer cells

HIGHLIGHTS

• who: Souvik Chakraborty from the , on glass slides with mounting media containing DAPI for fluorescence imaging using an Olympus , microscopeFluorescence images were background subtracted, and the fluorescence intensity of collagen-I immunoreactivity was analysed on a cell-by-cell basis for computing the average fluorescence intensity based on the analyses of several randomly selected fields containing, cells pooled from two independent experiments. Protein extraction, immunoblotting Total cell lysate from D monolayer culture was prepared by extracting cells with modified RIPA buffer (, mM Tris-HCl, pH, .5, mM NaCl, % NP-40, .25% sodium deoxycholate, .1% SDS, mM EDTA . . .
  

   

  

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