Prohormone convertase 1/3 deficiency causes obesity due to impaired proinsulin processing

HIGHLIGHTS

• who: Daniel T. Meier from the u03b2-cell mass Tissue sections from three to four different depths of the pancreas (at least , u03bcm apart) were prepared as described abovePancreas sections were imaged using an automated Eclipse Ni microscope (Nikon, Egg, Switzerland) with an Orca Flash, ., camera (Hamamatsu Photonics, Solothurn, Switzerland), a, u00d7 Objective NA, ., (Nikon), and a , slide loading robot (PRIOR Scientific, Fulbourn, UK) using NIS Elements software v5. Ilastik software, was trained to segment the pancreas area of stitched multichannel images, including channels for nuclei (DAPI), autofluorescence (GFP), lymph nodes (Alexa-555), and insulin (Alexa-647 . . .
  

   

  

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