Prolonged cigarette smoke exposure alters mitochondrial structure and function in airway epithelial cells

HIGHLIGHTS

• who: Hoffmann et_al and collaborators from the by trypsinCigarette smoke extract (CSE) was prepared fresh (used within , hr) from filter-less research-grade cigarettes ( R F, Tobacco Research Institute, University of Kentucky, Lexington, KY) as described previously [5]. BEAS- B cells were cultured in medium without, % (control), %, .5%, % or, .5% CSE for , months. For, % CSE and, .5% CSE the concentration was increased stepwise by, .5-1% after each passage every, days starting from, .5% CSE. Additionally, cells were exposed to, % CSE for , months followed by , months culture in, % CSE (referred to as, % CSE). Cell viability was tested . . .
  

   

  

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