HIGHLIGHTS
SUMMARY
The authors demonstrated that in_vitro, in crowded environments, purified P protein alone triggers LLPS. The domains of P required for phase separation in_vitro are the same as those identified in the cellular minimal system. In_vitro, in crowded environments, purified N0-P complexes also form liquid droplets, which are disrupted when incubated with RNA molecules. RABV P alone phase separates in_vitro in crowded environments Taken together, these data demonstrate that P alone, at low concentration, induces LLPS in_vitro in crowded environments. P CTD but not P NTD is required for P phase separation in_vitro The . . .
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