Purification, characterization, and preliminary serial crystallography diffraction advances structure determination of full-length human particulate guanylyl cyclase a receptor

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  • who: Shangji Zhang from the Center for AppliedState University Council (CSIC), Madrid, Spain have published the research work: Purification, characterization, and preliminary serial crystallography diffraction advances structure determination of full-length human particulate guanylyl cyclase A receptor, in the Journal: Scientific Reports Scientific Reports
  • how: These results indicated that solubilized pGC-A was well-captured in the affinity column. The Coomassie blue-stained results showed that the majority of contaminant proteins were washed off with the purification buffer containing 50 mM imidazole (Fig 3 lane 10). The western blotting results indicated that the single band . . .

     

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