Purification of an eh domain-binding protein from rat brain that modulates the gating of the rat ether-a̠-go-gochannel*

HIGHLIGHTS

• who: Elemer T. Piros from the Subcloning-The full-length cDNA encoding rat EAG (from DrOlaf Pongs) was subcloned into the KpnI and NotI restriction sites of pc, (Invitrogen). NH2- and COOH-terminal fragments (, and , base pairs, respectively) of EAG were generated using polymerase chain reaction. These fragments were subcloned into pc, for use in in vitro translation. Both NH2- and COOH-terminal constructs were confirmed by DNA sequencing. Rat epsin cDNA in pcDNA3.1/His B (Invitrogen) was obtained from Hong Chen and Pietro De Camilli (Yale University) and used in our transfection studies. Overlay Assay . . .
  

   

  

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