Purification of the full-length, membrane-associated form of the antiviral enzyme viperin utilizing nanodiscs

HIGHLIGHTS

• who: Ayesha M. Patel from the DepartmentUniversity of have published the research work: Purification of the full-length, membrane-associated form of the antiviral enzyme viperin utilizing nanodiscs, in the Journal: Scientific Reports Scientific Reports
• what: We describe, for the first time, the purification of full-length human viperin, rather than its truncated form, from a transfected mammalian cell line.
• future: Experiments are needed to better understand what additional factors contribute to viperin`s enzymatic activity.

SUMMARY

For DNA transfection in HEK293T_cells, transfection grade linear polyethylenimine (PEI) hydrochloride was . . .

 



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