Quantitative analysis of crispr/cas9-mediated provirus deletion in blue egg layer chicken pgcs by digital pcr

HIGHLIGHTS

• who: Stefanie Altgilbers from the Department have published the paper: Quantitative analysis of CRISPR/Cas9-mediated provirus deletion in blue egg layer chicken PGCs by digital PCR, in the Journal: Scientific Reports Scientific Reports
• how: As control for the PCR 1F/2R the authors used a nullizygous blue-allele PGC line. For the experiments the authors used a homozygous blue-allele bearing cell line which was established from this intercross in previous u00adwork39.

SUMMARY

Considering that the authors transfected homozygous blue-allele bearing PGCs, the appearance of an amplicon in PCR . . .

 



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