Rap80 is an independent prognosis biomarker for the outcome of patients with esophageal squamous cell carcinoma

HIGHLIGHTS

• who: Qingyuan Yang from the The shRNAs against different sequences of , (sh, #1, #2) were kindly presented by ProfZhenkun Lou lab at Mayo Clinic. The control shRNAs (shCon.) were purchased from Invitrogen. The HA-, plasmids were given by Prof. Ping Wang lab at Tongji University. The eukaryotic , overexpressing plasmids (Flag-RAP80) were constructed by amplifying the open reading frame (ORF) of human , from cDNA and ligated into the vector pCMV-N-Flag (Flag). The establishment of stable , knockdown EC cells and the transfection of plasmids were performed according to our previous protocols28. Antibodies have published the research . . .
  

   

  

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