Rapid differentiation of soil and root microbiomes in response to plant composition and biodiversity in the field

HIGHLIGHTS

• who: Haley M. Burrill from the Microbial community library preparation Bacterial, fungal, oomycete, and AM fungal communities were sequenced from both soil and root DNAFor all communities, we used a two-step PCR process: the first PCR reactions use community-specific primers to amplify those regions of rDNA, followed by a clean-up using AMPure XP beads (Beckman Coulter, Brea, CA, USA), then a second PCR to bind unique barcode combinations using Nextera XT Index Kit v, (Illumina, San Diego, CA, USA), and finally another AMPure XP bead clean-up. Following each PCR, PCR product were checked . . .
  

   

  

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