Rapid induction and long-term self-renewal of neural crest-derived ectodermal chondrogenic cells from hpscs

HIGHLIGHTS

• who: Pei Shen from the Cells and culture conditions hESCs, cells (passages, ), Hues, cells (passages, ), cells (passages, ), Hues, cells (passages, ) and iPSCs (ATCC-DYR0100, passages, ) were cultured in , medium on, % Matrigel (growth factor reduced, BD Biosciences)-coated disheshPSCs were passaged using Accutase (Millipore) at a dilution of, :10. Blebbistatin (Selleck, u03bcM) was used to improve cell attachment after passaging but was not used in the routine culture of hPSCs. Human preadipocytes (BFN608007090, Shanghai Cell Bank, passages, ), human osteoblasts (hFOB1.19, Shanghai Cell Bank, passages, ) were cultured in DMEM high glucose +, % FBS +, % Penicillin/Streptomycin (Gibco), and both were . . .
  

   

  

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