Rational design of lipase rol to increase its thermostability for production of structured tags

HIGHLIGHTS

  • who: Jeng Yeong Chow and Giang Kien Truc Nguyen from the (UNIVERSITY) have published the Article: Rational Design of Lipase ROL to Increase Its Thermostability for Production of Structured Tags, in the Journal: (JOURNAL) of 18/02/2022

SUMMARY

    Since the ROL_4x mutant (E190C/V209L/E238C/D262G) has a higher thermostability than ROL_WT, the single mutants were created3 based on the ROL_4x Int. Mutants and Thermostability Assays ROL_WT and the three mutants (ROL_4x, ROL_7x, ROL_10x) were subcloned and expressed in the pFAi2 vector containing To confirm thatanthe ROL mutants fromyield theand screening haveofincreased . . .

     

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