Re-evaluating human cytomegalovirus vaccine design: prediction of t cell epitopes

SUMMARY

    Of 17 To illustrate this imperative, the authors re-examined the question of optimal HCMV epitopes for T_cells by considering the hypothesis that HCMV proteins infrequently recognized by T_cells may contain targets that favor protection from transmission, infection, or disease. PBMCs stimulated with these peptide pools were gated for CD4+ /interferon (IFN)-γ+ /CD69+ or CD8+ /IFN-γ+ /CD69+ T_cells and for memory T_cell subsets (CD4+ /CD45RO+ /CD27+ or CD8+ /CD95+ ) (results summarized in Table S2 of_[55]). Excluding all others, proteins with no/few CD4 T_cell and no/few CD8 T_cell responders were included in the "Test Set . . .

     

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