Re-examining extreme carbon isotope fractionation in the coccolithophore ochrosphaera neapolitana

HIGHLIGHTS

• who: Hongrui Zhang from the Laboratory culture Ochrosphaera neapolitana (RCC1357) was precultured in K/, medium without Tris buffer, using artificial seawater (ASW) supplemented with Na, and HCl to yield an initial DIC of , u00b5MIn triplicate, L bottles were filled with , mL of seawater medium with air in the bottle headspace and inoculated with a mid-log phase preculture at an initial cell concentration of , cells mL-1. Cultures were grown at , u00b0C under a warm white LED light at , u00b1 , u00b5E on a, h-light/8h-dark cycle. Bottles were orbitally shaken at , rpm to keep cells . . .
  

   

  

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