Real time, spatial, and temporal mapping of the distribution of c-di-gmp during biofilm development*

HIGHLIGHTS

• who: Harikrishnan A.S. Nair from the SpeedVac (CentriVap威 benchtop vacuum concentrators) coupled with a CentriVap Cold Trap (Labconco Corp, series) until the volume reached ⬃, ␮l. The extracted mixture was then cooled to ⫺, °C and freeze-dried overnight (Labconco FreeZone, .5-liter benchtop freeze dry system). The dried extract was dissolved in , ␮l of , mM ice-cold ammonium acetate and centrifuged at, ⫻ g for , min at , °C. The supernatant was then transferred to HPLC vials and analyzed by LC-MS/MS (NUS-Environmental Research Institute at the National University of Singapore). LC-MS/MS-Quantification of c . . .
  

   

  

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