HIGHLIGHTS
SUMMARY
Detection and analysis of proteins and nucleic_acids from human fluids are essential for disease and environmental monitoring, public health, and clinical diagnostics. PCR and ELISA are considered the "gold standards" for nucleic_acid and protein detection, respectively. Of 30 To date, digital biosensing has significantly improved detection limits for immunoassays and nucleic_acid tests to the single molecule scale. Successfully translating conventional biosensing technology to droplet biosensing for nucleic_acid and protein detection requires careful consideration of the biological processes and technical requirements for the assays. In this review, the authors analyze the current state of digital . . .
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