Receptor activity-modifying protein-directed g protein signaling specificity for the calcitonin gene-related peptide family of receptors*

HIGHLIGHTS

• who: Cathryn Weston from the Materials-Human (h) ␣CGRP, hAM, and h, (1-, ) were purchased from Bachem (Bubendorf, Switzerland) and made to , mM stocks in water containing, % BSAYeast nitrogen base and yeast extract were purchased from Difco (Franklin Lakes, NJ). Fluorescein-di-␤-D-glucopyranoside was purchased from Invitrogen. Forskolin was from Tocris Bioscience (Wiltshire, UK), and , was supplied by Alpha Laboratories (Hampshire, UK). Both the ALPHAScreen and LANCE威 cAMP detection assay kits and all reagents were from PerkinElmer Life Sciences. Expression Constructs-To enable expression of the human CLR, we used either a previously described (25) Myc . . .
  

   

  

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