Reciprocal regulation of notch and nuclear factor of activated t-cells (nfat) c1 transactivation in osteoblasts*

HIGHLIGHTS

• who: Stefano Zanotti from the EXPERIMENTAL PROCEDURES Expression Vectors-A, kilobase (kb) DNA fragment containing the murine NICD coding sequence (J. S. Nye, Columbia University, New York) was cloned into either pcDNA, ., (Invitrogen) for use in acute transfection experiments, or into the retroviral vector pLPCX (Clontech, Palo Alto, CA) for the creation of stably transduced cell lines (35)., and, .1-kb DNA fragments containing the respective coding sequences of murine Hey, and Hey, (T. Iso, University of Southern California, Los Angeles, CA) were cloned into pcDNA, .1, and used in acute transfection experiments (13, ). A, .3-kb . . .
  

   

  

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