Recovering individual haplotypes and a contiguous genome assembly from pooled long-read sequencing of the diamondback moth (lepidoptera: plutellidae)

HIGHLIGHTS

SUMMARY

    The authors trialed 2 postassembly haplotype merging procedures, purge_dups and Haplomerger 2. 12, No 10 (a) canu flye wtdbg 5e+08 ID 4e+08 canu +HM2 +HiC canu +purge_dups +HiC 3e+08 flye flye +HM2 +HiC 2e+08 flye +purge_dups +HiC wtdbg wtdbg +HM2 +HiC 1e+08 wtdbg +purge_dups +HiC 0e+00 0 (b) %BUSCO genes 75 Complete and duplicated BUSCOs (D) 50 Complete and single-copy BUSCOs (S) Fragmented BUSCOs (F) Missing BUSCOs (M) canu HM2 HiC ef. The authors find that "canu - purge_dups" produced the lowest final N50 value, whereas all other . . .

     

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