Recq family members combine strand pairing and unwinding activities to catalyze strand exchange*

HIGHLIGHTS

• who: Amrita Machwe from the Enzymes-WRN is the only human RecQ helicase that has been demonstrated to have, ⬘ to, ⬘ exonuclease activity in addition to its requisite DNA unwinding activity (23, )The WRN-E A mutant contains a glutamate to alanine substitution in the conserved nuclease domain that completely abolishes the inherent exonuclease activity of WRN, whereas its helicase activity is not affected (23, ). Wild-type WRN and WRN-E A proteins were individually overproduced by baculoviral infection of Sf, insect cells and purified by a three-step chromatography procedure described previously (26), with the exception that . . .
  

   

  

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