Reducing seed shattering in weedy rice by editing sh4 and qsh1 genes: implications in environmental biosafety and weed control through transgene mitigation

HIGHLIGHTS

SUMMARY

    In the case of weedy rice, reduced seed shattering is a domesticated characteristic that is fatal for the weeds, depending on natural seed shattering for self-reproduction, but beneficial for cultivated rice, for which seed persistence is critical for the grain harvest. A previous study successfully developed two GE rice lines containing artificial microRNA genes by silencing the rice SH4 gene, a QTL (quantitative trait locus) responsible for seed shattering in cultivated rice. Whether it is possible to edit the seed-shattering genes, including SH4, in weedy rice to develop substantially reduced seed-shattering . . .

     

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