Reduction in water activity greatly retards the phosphoryl transfer from atp to enzyme protein in the catalytic cycle of sarcoplasmic reticulum ca-atpase (∗)

HIGHLIGHTS

• who: Hiroshi Suzuki from the (UNIVERSITY) have published the paper: Reduction in Water Activity Greatly Retards the Phosphoryl Transfer from ATP to Enzyme Protein in the Catalytic Cycle of Sarcoplasmic Reticulum Ca-ATPase (∗), in the Journal: (JOURNAL) of November/27,/1995
• what: The authors have investigated the effects of organic solvents (Me2SO, DMF, and glycerol) on the conformational change in the calcium-enzyme-substrate complex and EP formation from ATP by using EDANS-labeled SR vesicles.
• how: Similar results were obtained with 30% (v/v) DMF (Fig 1C) or 30% (v/v) glycerol . . .
  

   

  

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