Refinement of microbiota analysis of specimens from patients with respiratory infections using next-generation sequencing

HIGHLIGHTS

• who: Hiroaki Ikegami from the DepartmentUniversity of have published the Article: Refinement of microbiota analysis of specimens from patients with respiratory infections using next-generation sequencing, in the Journal: Scientific Reports Scientific Reports
• what: The authors investigated the optimal threshold for clustering 16S rRNA gene sequences into OTUs using NGS technology (MiSeq sequencer; Illumina) by comparing the results with those obtained using the Sanger method.

SUMMARY

Streptococcus pseudopneumoniae (37.8%) was the most abundant phylotype, followed by H. influenzae (13.3%) in NGS technology (99% and 97% identity thresholds for clustering . . .

 



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